Plant Molecular Farming: A Viable Platform for Recombinant Biopharmaceutical Production

COST Action FA0804 Molecular Farming: Plant as a Production Platform for High Value Proteins

α-Mannosidosis is a rare lysosomal storage disease with autosomal recessive inheritance that leads to mental and physical deterioration. This pathology is due to progressive accumulation of undegraded oligosaccharides inside lysosomes. The deficiency of the α-mannosidase (LAMAN) enzyme, which normally cleaves α-linked mannose residues from glycoproteins during their ordered degradation, is the ...

Assessing the bioconfinement potential of a Nicotiana hybrid platform for use in plant molecular farming applications

BACKGROUND The introduction of pharmaceutical traits in tobacco for commercial production could benefit from the utilization of a transgene bioconfinement system. It has been observed that interspecific F1Nicotiana hybrids (Nicotiana tabacum × Nicotiana glauca) are sterile and thus proposed that hybrids could be suitable bioconfined hosts for biomanufacturing. We genetically tagged hybrids with...

Advances in plant molecular farming.

Plant molecular farming (PMF) is a new branch of plant biotechnology, where plants are engineered to produce recombinant pharmaceutical and industrial proteins in large quantities. As an emerging subdivision of the biopharmaceutical industry, PMF is still trying to gain comparable social acceptance as the already established production systems that produce these high valued proteins in microbia...

Plant Breeding and Molecular Farming

A Cost-Effective ELP-Intein Coupling System for Recombinant Protein Purification from Plant Production Platform

BACKGROUND Plant bioreactor offers an efficient and economical system for large-scale production of recombinant proteins. However, high cost and difficulty in scaling-up of downstream purification of the target protein, particularly the common involvement of affinity chromatography and protease in the purification process, has hampered its industrial scale application, therefore a cost-effectiv...